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Absorbance at 615 nm and/or fluorescence at 630 nm were measured in triplicates, including separate blanks for every concentration of the reagents

Exposure of immature mouse oocytes at the germinal vesicle stage (GV oocytes) to H 2 O 2 increases levels of reactive aldehydes, including 4-hydroxynonenal (4-HNE), which modifies and damages -, -, and -tubulins, essential for spindle assembly

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Duperray, J., Sergheraert, R., Chalothorn, K., Tachalerdmanee, P., & Perin, F